FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008122959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52222284
Sequences flagged as poor quality0
Sequence length154
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG914951817.52033289084024No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGTAGATC5057980.9685482159301956Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG2950030.5648986934389925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT2244510.4297992787906404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG1815470.34764278023534934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG1710980.3276340805009601No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGTAGATCTCGG1392930.2667309610586929Illumina Single End PCR Primer 1 (96% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG1391500.26645713159539325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG1382040.26464564437664195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG1374350.2631730929271497No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGTAGATCTCGGTG1274300.24401460495293545Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGTAGATCT1120700.2146018737901238Illumina Single End PCR Primer 1 (96% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1062730.20350124862405483Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG763480.14619812492306922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG677990.12982771875699656No Hit
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGTA654560.12534112831985672Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCGCATATGTGT652480.12494283091869364Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG581780.1114045490618526No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)