FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008122971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29832142
Sequences flagged as poor quality0
Sequence length154
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG465755315.612532951874524No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1279750.42898361103269084Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTAGATC991710.33243003469211163Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG913830.3063239642664613No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTAGATCT820840.2751528871108216Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGA705090.23635245501311977Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT683210.22901808391767509No Hit
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTA589820.19771292319539108Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG557740.18695942115051611No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTG529330.17743613582960283Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGT515910.1729376321686857Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG471400.15801748329033832No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGT466530.15638501586644366Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG451990.1515110782189224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG417780.1400435811816664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG415630.13932288201095316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG408510.13693619452468414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG386380.1295180212000868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG371130.12440608522177186No Hit
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTAG352050.11801029909283751Illumina Single End PCR Primer 1 (97% over 34bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTAGATCTCGGTG350960.11764492137373173Illumina Single End PCR Primer 1 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG350880.11761810466040287No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGCCTGAGTGTAGA334630.11217095976547711Illumina Single End PCR Primer 1 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)