FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008122984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19727367
Sequences flagged as poor quality0
Sequence length154
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGATCGGAAGAGCA781440.3961197660083071TruSeq Adapter, Index 10 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG579670.2938405312781985Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGACTC339480.1720858135806973TruSeq Adapter, Index 10 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGACTCT207480.10517369094415895TruSeq Adapter, Index 10 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)