FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008122986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16801115
Sequences flagged as poor quality0
Sequence length154
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3712442.209639062645545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAG520120.3095746919177686TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAGCC260370.15497185752255133TruSeq Adapter, Index 5 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAGC208570.12414057043237904TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAGCA202160.12032534745461834TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAGCT198860.11836119209945294TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTAGCG173910.10351098721721744TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)