FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008122989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008122989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24849209
Sequences flagged as poor quality0
Sequence length154
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17508897.046055268801514No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4419411.778491218774811Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG797080.3207667495572998Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG495880.19955564782766327No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGTGTAGATC470850.1894828925942874Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG421610.16966737251073064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG403270.16228685589146924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG394550.15877768986529914No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGT354230.14255182126722826Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGTG326880.13154543470578883Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG318060.12799602594996082No Hit
TCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGTGTAGA308660.12421320936211691Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT299980.12072014042780999No Hit
CGTGTGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG271210.10914230710522817Illumina Single End PCR Primer 1 (100% over 30bp)
CGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT267350.1075889377404327Illumina Single End PCR Primer 1 (100% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGTGTAGAT264470.10642994712628479Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCGGTGTAGA249260.10030902794531608Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)