FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43446699
Sequences flagged as poor quality0
Sequence length154
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGGCGACCACCGAGATCTACACGTTGACCTACACTCTTTCCCTACA848900.1953888372509037Illumina RNA PCR Primer (96% over 31bp)
ACACGTTGACCTACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAA755780.17395567842795145Illumina Single End PCR Primer 1 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG607700.13987253669145266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACGTTGACCTACACTCTTTCCCTACACGACGCTCTTCAGATCGGAAGA461300.10617607565536796Illumina Single End PCR Primer 1 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)