FastQCFastQC Report
Fri 1 Sep 2023
EGAF00008123017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34615523
Sequences flagged as poor quality0
Sequence length154
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG614593017.75483790899245No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGATC2639530.7625278404720333Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGT1975220.5706168299118288Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1819840.5257294538060281No Hit
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTA1560930.45093353060128544Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT1284070.3709520725716032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG1209240.34933460343788536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG1115260.32218493419845196No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGATCTCGG1091970.3154567388740595Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGATCT998100.28833884728536385Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG974900.2816366518570296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG947280.273657572644504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG945260.27307401942186454No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGATCTCGGTG941600.272016690315498Illumina Single End PCR Primer 1 (96% over 27bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAG816000.23573239092761938Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGAT725960.20972093936006686Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGT656840.18975301918737442Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG517350.14945606917451457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG474730.13714367395229016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG398310.1150668733215442No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTGTAGA387420.11192088589850283Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGACGTTGTG376190.10867667664590824Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG348120.10056759795309175No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)