FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008123019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28286147
Sequences flagged as poor quality0
Sequence length154
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22420327.926254501894514No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5234731.8506338102534783Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGT833340.29461064456746267Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGTAGATC810330.2864759205274582Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGT733570.25933896193072886Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGTAG692450.24480181058240277Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATA626030.22132035161946942Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTG621780.2198178493521935Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGTA542730.19187130718086137Illumina Single End PCR Primer 1 (96% over 33bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGTAGAT514300.18182045083764856Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG429380.15179868788774942No Hit
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAG374050.13223787601754314Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT353300.12490212965378424No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCGATAGTGTAGA283300.10015503348688672Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)