FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44107944
Sequences flagged as poor quality0
Sequence length154
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17363983.9367012890013644No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2015450.456935829972034Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG805880.1827063170298756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG761600.1726673090906255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG608530.13796380987515536No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATTGCGGTGT471700.10694218710352947Illumina Single End PCR Primer 1 (97% over 37bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATTGCGGTGTAGATC449370.10187960699324365Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT444480.10077096316255413No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)