FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008123045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6480950
Sequences flagged as poor quality0
Sequence length154
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4001586.174372584266195No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG173460.2676459469676513Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACATGCCAGTGT113270.1747737600197502Illumina Single End PCR Primer 1 (97% over 37bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACATGCCAGTGTA106950.1650221032410372Illumina Single End PCR Primer 1 (97% over 37bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACATGCCAGTGTAGATC97520.1504717672563436Illumina Single End PCR Primer 1 (97% over 37bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACATGCCAGT74280.11461282682322807Illumina Single End PCR Primer 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)