FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38766680
Sequences flagged as poor quality0
Sequence length154
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4188371.0804046155100204Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTACGC783150.20201626757823987TruSeq Adapter, Index 21 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTACGCA737580.19026132751115132TruSeq Adapter, Index 21 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTACGCAA414090.10681595638316205TruSeq Adapter, Index 21 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)