FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008123083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18398186
Sequences flagged as poor quality0
Sequence length154
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7394524.019157106032084No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2936761.5962225841178037Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGTGT605750.3292444157266374Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGTGTA547190.29741519082370405Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGTGTAG458370.24913869226020433Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGT325920.17714789925485044Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATG315770.1716310510177471Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGCCATGGTG303800.16512497482088723Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG238120.1294258031742912No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT199560.10846721519175857Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG199050.10819001395028836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG191160.10390154768519028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)