FastQCFastQC Report
Sat 11 May 2024
EGAF00008123092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18440700
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length154
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1113080.6035996464342459Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTTCA300060.16271616587222826TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTTCAGC277390.15042270629639873TruSeq Adapter, Index 8 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTTCAG251440.1363505723752352TruSeq Adapter, Index 8 (97% over 37bp)
ACACGCACAACTACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAA214050.1160747693959557Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)