FastQCFastQC Report
Thu 9 May 2024
EGAF00008123093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18440700
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length154
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4981562.7013941987017844No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG945050.512480545749348Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTGT651750.3534301843205518Illumina Single End PCR Primer 1 (97% over 37bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTGTA421470.22855423058777596Illumina Single End PCR Primer 1 (97% over 37bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTGTAGATC359630.1950197118330649Illumina Single End PCR Primer 1 (97% over 37bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGT294850.15989089351271915Illumina Single End PCR Primer 1 (97% over 37bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTG283780.153887867597217Illumina Single End PCR Primer 1 (97% over 37bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGCGTGTAG272500.14777096314131244Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG258090.13995672615464708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG238160.12914911039168794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG217250.11781006144018394No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTGTGC206020.1117202709224704Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT185640.10066862971579171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)