FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38024709
Sequences flagged as poor quality0
Sequence length154
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17145254.508975992426398No Hit
AGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGGATTCGTGTAG1164460.3062377150604887Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGGATTCGTGTAGATCT681460.1792150467213306Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG679410.17867592359483933No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG592620.15585129132743658Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG500260.13156182207732345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT485420.12765909661530875No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)