FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42223794
Sequences flagged as poor quality0
Sequence length154
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG22783635.395922024439584Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTT3566580.8446848712837127TruSeq Adapter, Index 2 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTA2227390.5275200992123067TruSeq Adapter, Index 2 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTAC1309060.31002898507888704TruSeq Adapter, Index 2 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTAT1010180.23924425171267175TruSeq Adapter, Index 2 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTAA939380.2224764548633408TruSeq Adapter, Index 2 (97% over 39bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGT873780.2069401911159381TruSeq Adapter, Index 2 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTTAG740190.17530163206082333TruSeq Adapter, Index 2 (97% over 39bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACG714810.16929080319025808TruSeq Adapter, Index 2 (97% over 38bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG624090.14780528722738653TruSeq Adapter, Index 2 (100% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT601280.14240311990912044Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAC471310.11162189735957882TruSeq Adapter, Index 4 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA456850.10819728800306291TruSeq Adapter, Index 2 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)