FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42223794
Sequences flagged as poor quality0
Sequence length154
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27048246.405923636326949No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20582474.874614062393351Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGAT1929600.4569935141309187Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1104760.26164394417043624No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGT1063950.2519787776531877Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTG970800.22991775679845353Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGTAGATC899250.21297233498249823Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATG853960.20224615533128076Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG846540.20048885232814467Illumina Single End PCR Primer 1 (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT794220.18809773465643567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG755240.17886597305775034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG630510.14932575694169029No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG630360.1492902319483654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG621650.14722741400263556No Hit
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGTAG617140.14615929587000165Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG588620.13940481047250278No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGGG531870.12596452133126643Illumina Single End PCR Primer 1 (96% over 30bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGTAGAT529040.12529428312387086Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT516060.12222018703482686Illumina Single End PCR Primer 1 (100% over 31bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGTAGA515410.12206624539708583Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGTA470730.11148453405205605Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTGGATGTGT462960.10964433939782862Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG433020.10255355073018782Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)