FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62711814
Sequences flagged as poor quality0
Sequence length154
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41969406.692423217099094No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5179610.8259384746867632Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGTGTAGATC1106160.176387817453343Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGT989420.15777250519335959Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG954870.15226317644072615No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGTG847610.13515954107147976Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGTGT739600.11793631101151053Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA720990.1149687680857071Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG715760.11413479444239966No Hit
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGTGTA696610.11108114333927577Illumina Single End PCR Primer 1 (97% over 36bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAGTGTAG650190.10367902928146841Illumina Single End PCR Primer 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)