FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008123125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008123125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73678272
Sequences flagged as poor quality0
Sequence length154
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44173455.995451413409913No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1509040.2048147926162003Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1483900.2014026604749905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT1059340.14377915920720832No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAGCGTAGTGT902110.12243908217608579Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAGCGTAGTGTA856250.11621472338547788Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG824060.11184572841230586No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAGCGTAGTGTAGATC803470.10905114604207874Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG799900.108566606991No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)