FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7152
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC578380.85850111856824No Hit
AGCACTTGAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1592.2231543624161074No Hit
AGCACTTGAATCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1311.83165548098434No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC761.0626398210290828No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC570.7969798657718121No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.4893736017897092No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC340.47539149888143173No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC330.4614093959731544No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC320.44742729306487694No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.39149888143176736No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.33557046979865773No Hit
AGCACTTGAATCAACAGTGATCAGCACTATCGAGACCAAGTCTCTGCTAC210.2936241610738255No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC200.2796420581655481No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC190.2656599552572707No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC180.25167785234899326No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC170.23769574944071586No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC170.23769574944071586No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC160.22371364653243847No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC150.20973154362416108No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC150.20973154362416108No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.18176733780760626No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC130.18176733780760626No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC130.18176733780760626No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.18176733780760626No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC120.16778523489932887No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.16778523489932887No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC110.15380313199105144No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC110.15380313199105144No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC110.15380313199105144No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.12583892617449663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC90.12583892617449663No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC90.12583892617449663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC90.12583892617449663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC80.11185682326621924No Hit
AGCACTTGAATCAATAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC80.11185682326621924No Hit
AGCACTTAAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC80.11185682326621924No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)