FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239
Total Bases34.6 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA9740.58577405857741No Hit
TGCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA6828.451882845188287No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC197.949790794979079No Hit
TGCTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA93.765690376569038No Hit
TGCTGATCATTGTTGCTTTAAGTGCTACACCTTGTAGAACCATGTCGTCA41.6736401673640167No Hit
TGCTACACCTTGTTGCACCATGTCGTCAGTGTAGATCTCGGTGGTCGCCG10.41841004184100417Illumina Single End PCR Primer 1 (95% over 24bp)
TGCTGATCATTGTTGCTTCGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC10.41841004184100417No Hit
TGTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATGAGACAAGTAAACCCCGCCACGTATCCCCTCGTCACCGCCGTG10.41841004184100417No Hit
TGCTGATTATTGTTGGTTTGGGCAGCCTGTTGAATCAAGTGCTACACCTT10.41841004184100417No Hit
TGCTGATCATTGTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT10.41841004184100417No Hit
TCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGGGTATTTCCTC10.41841004184100417No Hit
TGCTGATGATTGTTGCTTCAAGTGCTACACCTTGTTGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGACATTGTTGATTCAAGTGCTACACCTTGTAGAAGCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGGTGCTTCTAGTGCTACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGCACCATGTCGTAA10.41841004184100417No Hit
TGCTGATCATTGTTGATTTGGGGGTGAGGAAAAGACTGGAGTATTTGCTC10.41841004184100417No Hit
TGCTGATCATTGTTGCTTCAAGTGCTAAACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGACATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGATACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTGGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGCGTCAG10.41841004184100417No Hit
TGATGATCCGGTGGGCGTTGTGGGGGCGTCGCAGGAGGTTACGTGAGGAC10.41841004184100417No Hit
TCGTAAAAATTTTTAATAAAATTCAAACCCATTTAAAAACAATTACTAAA10.41841004184100417No Hit
TGCTGATCATTGTTGTTTTAAGTGCTACACCTTGTAGAACCAAGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGAATGGAGTATTTCCTC10.41841004184100417No Hit
TGCTGATGATTGTTGATTCAAGTGCTAGACCTTGTAGAACCATGTCGTCT10.41841004184100417No Hit
TGCTGATCATTGTTGCTTCAAGTGATACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGCTTCAGTGCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC10.41841004184100417No Hit
TGCTGATGATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGACGACA10.41841004184100417No Hit
TGCTGATTATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATTTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTAAGTGCTACACCATGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGTTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGGTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT10.41841004184100417No Hit
TGCTGATCATTGGTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA10.41841004184100417No Hit
TGCTGATCATTGTTGCTTCAAGTCTACACCTTGTAGAACCATGTCGTCAG10.41841004184100417No Hit
TGCTGATGATACAAGGAAAGACCGTATGTCACACCGAATAAACCGCGTCT10.41841004184100417No Hit
TGCTGATCATTGTTGCTTTGGGCGTGCGGAAAACTCAGGAGTATTTCCTC10.41841004184100417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)