FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7344
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC591780.56917211328975No Hit
AGCACTTGAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1171.5931372549019607No Hit
AGCACTTGAATCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1031.4025054466230935No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC720.9803921568627451No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC560.7625272331154684No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC470.6399782135076253No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC450.6127450980392157No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC360.49019607843137253No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.3812636165577342No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC260.3540305010893246No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC250.3404139433551198No Hit
AGCACTTGAATCAACAGTGATCAGCACTATCGAGACCAAGTCTCTGCTAC250.3404139433551198No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC250.3404139433551198No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC230.31318082788671026No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC220.2995642701525054No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC210.28594771241830064No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC200.27233115468409586No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC190.2587145969498911No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.24509803921568626No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC170.23148148148148145No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.23148148148148145No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.23148148148148145No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC160.2178649237472767No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC150.20424836601307192No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC150.20424836601307192No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC130.1770152505446623No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC130.1770152505446623No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC130.1770152505446623No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC120.16339869281045752No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC120.16339869281045752No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.16339869281045752No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC110.1497821350762527No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC110.1497821350762527No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC100.13616557734204793No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC100.13616557734204793No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC100.13616557734204793No Hit
AGCACTCGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.13616557734204793No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC100.13616557734204793No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC90.12254901960784313No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACACACAC90.12254901960784313No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.12254901960784313No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC90.12254901960784313No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.10893246187363835No Hit
AGCACTTGAATCAATAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC80.10893246187363835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)