FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8508
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT463654.489891866478615No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC175720.651151857075696No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA881.0343206393982134No Hit
TGCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA800.9402914903620121No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC360.4231311706629055No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC290.3408556652562294No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.31734837799717913No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT260.305594734367654No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC250.29384109073812886No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT250.29384109073812886No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT240.2820874471086037No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC220.25858015984955335No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAGAAGTCTGGAGTATTTCCT210.2468265162200282No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT210.2468265162200282No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.2468265162200282No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC200.23507287259050302No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC200.23507287259050302No Hit
TGCTGATCATTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC190.2233192289609779No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT190.2233192289609779No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC190.2233192289609779No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGGGTATTTCCT160.18805829807240243No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTCCCT160.18805829807240243No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT160.18805829807240243No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC160.18805829807240243No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT150.1763046544428773No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.1763046544428773No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.1763046544428773No Hit
TGCTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA140.16455101081335213No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC140.16455101081335213No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT140.16455101081335213No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.152797367183827No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.152797367183827No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.152797367183827No Hit
TGCTGATCAATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT120.14104372355430184No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT120.14104372355430184No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGGAAAGTCTGGAGTATTTCCT120.14104372355430184No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT120.14104372355430184No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.14104372355430184No Hit
TGCTGACATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12929007992477667No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTGTTTCCT100.11753643629525151No Hit
TGCTGATCATTGTTGCTTTGGGGAGTGAGGAAAAGTCTGGAGTATTTCCT90.10578279266572638No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCC90.10578279266572638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)