FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7073
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC541976.61529761063198No Hit
TGCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA841.1876148734624628No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA711.0038173335218437No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT450.6362222536406051No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.5938074367312314No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.4241481690937367No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC280.39587162448748764No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC230.32518026297186486No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.3110419906687403No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.3110419906687403No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC210.2969037183656157No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC210.2969037183656157No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC190.26862717375936657No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.26862717375936657No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.25448890145624203No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT180.25448890145624203No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.25448890145624203No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC180.25448890145624203No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.24035062915311747No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC160.22621235684999294No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC160.22621235684999294No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC150.21207408454686835No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC140.19793581224374382No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC140.19793581224374382No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC140.19793581224374382No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.19793581224374382No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC130.18379753994061926No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC130.18379753994061926No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.18379753994061926No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.18379753994061926No Hit
TGCTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA120.1696592676374947No Hit
TGCTGATGATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.1696592676374947No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC120.1696592676374947No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC100.14138272303124558No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT100.14138272303124558No Hit
TGCTGATCCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.14138272303124558No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTAGAGTATTTCCTC90.12724445072812102No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.12724445072812102No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.12724445072812102No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC90.12724445072812102No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGACTGGAGTATTTCCTC90.12724445072812102No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.12724445072812102No Hit
TGCCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.11310617842499647No Hit
TACTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.11310617842499647No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC80.11310617842499647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)