FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7574
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC618281.6213361499868No Hit
AGCACTTGAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1061.399524689728017No Hit
AGCACTTGAATCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC841.1090573012939002No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC590.7789807235278585No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC570.7525745973065752No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC550.7261684710852918No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC470.6205439662001585No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC450.5941378399788751No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC270.35648270398732507No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC240.31687351465540003No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC220.29046738843411674No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC210.27726432532347506No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC210.27726432532347506No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC200.2640612622128334No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.2640612622128334No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC200.2640612622128334No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC180.23765513599155003No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.23765513599155003No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC180.23765513599155003No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.22445207288090838No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.2112490097702667No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC160.2112490097702667No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC150.19804594665962502No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC150.19804594665962502No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.1716398204383417No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC130.1716398204383417No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC130.1716398204383417No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC130.1716398204383417No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.1716398204383417No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC120.15843675732770002No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.15843675732770002No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC120.15843675732770002No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC120.15843675732770002No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC110.14523369421705837No Hit
AGCACTTGAATCAACAGTGATCAGCACTATCGAGACCAAGTCTCTGCTAC100.1320306311064167No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC100.1320306311064167No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.1320306311064167No Hit
AGCACTTAAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC100.1320306311064167No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC90.11882756799577501No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC90.11882756799577501No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC90.11882756799577501No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.11882756799577501No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC90.11882756799577501No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCTATTTTCAAACTCACAC90.11882756799577501No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.10562450488513335No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC80.10562450488513335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)