FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5784
Total Bases838.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC408070.53941908713693No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA2354.0629322268326415No Hit
TGCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA1883.250345781466114No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTAGAGTATTTCCTC490.84716459197787No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.7261410788381742No Hit
TGCTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA410.7088520055325035No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT250.4322268326417704No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC220.38035961272475793No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3457814661134163No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3457814661134163No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.3284923928077455No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.3112033195020747No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT160.2766251728907331No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC160.2766251728907331No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC160.2766251728907331No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC150.2593360995850622No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.24204702627939143No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.2247579529737206No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.2247579529737206No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC130.2247579529737206No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC130.2247579529737206No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.2247579529737206No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC120.2074688796680498No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.19017980636237897No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC110.19017980636237897No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.17289073305670816No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC100.17289073305670816No Hit
TGCTGATCATTGTTGCTTTGGAGGTGAGGAAAAGTCTGGAGTATTTCCTC90.15560165975103735No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC90.15560165975103735No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAATTCCTC90.15560165975103735No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.15560165975103735No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAAGAAAAGTCTGGAGTATTTCCTC90.15560165975103735No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTG80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCATC80.13831258644536654No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC80.13831258644536654No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC80.13831258644536654No Hit
TGCTGATCATTGTTGCTTTGGGGGTTAGGAAAAGTCTGGAGTATTTCCTC80.13831258644536654No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.12102351313969571No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC70.12102351313969571No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC70.12102351313969571No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC70.12102351313969571No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.12102351313969571No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.12102351313969571No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.12102351313969571No Hit
TGCTGATCATTGTTGCTTTGGGGGAGAGGAAAAGTCTGGAGTATTTCCTC60.1037344398340249No Hit
TGCTGATCATTGTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT60.1037344398340249No Hit
TGCTGATCATTGTTGCTTTAAGTGCTACACCTTGTAGAACCATGTCGTCA60.1037344398340249No Hit
GGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC60.1037344398340249No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.1037344398340249No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC60.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)