FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6181
Total Bases896 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC427469.14738715418217No Hit
TGCTGATCATTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA2684.335867982527099No Hit
TGCTGATCATTGTTGCTTCAAGTGCTACACCTTGTAGAACCATGTCGTCA1842.9768645850186055No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1832.9606859731435042No Hit
TGCTGATCACTGTTGATTCAAGTGCTACACCTTGTAGAACCATGTCGTCA360.5824300275036401No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.420643908752629No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.420643908752629No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.420643908752629No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.33975084937712347No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT200.3235722375020223No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC190.30739362562692124No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.25885779000161785No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC160.25885779000161785No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.22650056625141565No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC140.22650056625141565No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC130.2103219543763145No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.1941433425012134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC120.1941433425012134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTTGAGTATTTCCTC120.1941433425012134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC110.1779647306261123No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.1779647306261123No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1779647306261123No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1779647306261123No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC110.1779647306261123No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1779647306261123No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC100.16178611875101115No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC100.16178611875101115No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.14560750687591004No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGTCTGGAGTATTTCCTCT90.14560750687591004No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC90.14560750687591004No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC90.14560750687591004No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.14560750687591004No Hit
TGCTGATCATTGTTGTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.12942889500080892No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC80.12942889500080892No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC80.12942889500080892No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC80.12942889500080892No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.11325028312570783No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.11325028312570783No Hit
TGCTGATCATTGTTGCTTTAAGTGCTACACCTTGTAGAACCATGTCGTCA70.11325028312570783No Hit
TGCTGATCCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.11325028312570783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)