FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008124962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008124962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7653
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC612980.08624068992552No Hit
AGCACTTGAATCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC2313.018424147393179No Hit
AGCACTTGAAGCAACAATGATCAGCACTATCGAGACCAAGTCTCTGCTAC1361.7770808833137333No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC771.0061413824643932No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC580.7578727296485038No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC490.6402717888409775No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC460.6010714752384686No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.4181366784267607No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.4181366784267607No Hit
AGCACTTGAATCAACAGTGATCAGCACTATCGAGACCAAGTCTCTGCTAC280.36586959362341565No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC250.32666928002090684No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC230.3005357376192343No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC230.3005357376192343No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC230.3005357376192343No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.28746896641839803No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.28746896641839803No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.28746896641839803No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC200.26133542401672544No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.2482686528158892No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC150.1960015680125441No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC150.1960015680125441No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC140.18293479681170782No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC140.18293479681170782No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC130.16986802561087155No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC110.14373448320919902No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC110.14373448320919902No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC110.14373448320919902No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC100.13066771200836272No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC100.13066771200836272No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC100.13066771200836272No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC90.11760094080752645No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC90.11760094080752645No Hit
AGCACTTGAATCAATAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.11760094080752645No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGTGCCATTTTCAAACTCACAC80.10453416960669018No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.10453416960669018No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC80.10453416960669018No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC80.10453416960669018No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)