FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5708
Total Bases827.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT272047.65241765942537No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC113419.86685353889278No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA2534.432375613174492No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA1953.4162578836720394No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA540.94604064470918No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.47302032235459No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.3679046951646811No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT180.31534688156972673No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT160.2803083391730904No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT150.26278906797477225No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.26278906797477225No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.2452697967764541No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC140.2452697967764541No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.22775052557813596No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT120.21023125437981782No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.21023125437981782No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT120.21023125437981782No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT120.21023125437981782No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.21023125437981782No Hit
TGCTGATCATTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC120.21023125437981782No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.19271198318149965No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC110.19271198318149965No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.19271198318149965No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.17519271198318148No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.17519271198318148No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT100.17519271198318148No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT100.17519271198318148No Hit
TGCTGATCAATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT100.17519271198318148No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.17519271198318148No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGGGTATTTCCT90.15767344078486337No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.15767344078486337No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT80.1401541695865452No Hit
TGCTGACATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.1401541695865452No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGGAAAGTCTGGAGTATTTCCT80.1401541695865452No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT80.1401541695865452No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTCCCT80.1401541695865452No Hit
TGCTGATCATTGGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT70.12263489838822705No Hit
TGTTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT70.12263489838822705No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC70.12263489838822705No Hit
TGCTGATCATTGTTCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.12263489838822705No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT70.12263489838822705No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.12263489838822705No Hit
TGCTGATCATTGTTGCTTTGGGGGGGGAGGAAAAGTCTGGAGTATTTCCT60.10511562718990891No Hit
TGCTGATCATTGTTGTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC60.10511562718990891No Hit
TGCTGATCATTGTTGCTTTGGGAGGTGAGGAAAAGTCTGGAGTATTTCCT60.10511562718990891No Hit
TGCTGATCATTTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC60.10511562718990891No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGTGGAAAAGTCTGGAGTATTTCCT60.10511562718990891No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC60.10511562718990891No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.10511562718990891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)