FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590
Total Bases517.9 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC209358.30083565459609No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC41911.671309192200557No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC3419.498607242339833No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC892.479108635097493No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC471.309192200557103No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC230.6406685236768802No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.5571030640668524No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.44568245125348194No Hit
AGCACTTGAATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAGTGCGC140.38997214484679665No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.362116991643454No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.362116991643454No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.362116991643454No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC120.3342618384401114No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.3064066852367688No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC110.3064066852367688No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC100.2785515320334262No Hit
AGCACTTGAAGCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.25069637883008355No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.25069637883008355No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC90.25069637883008355No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.25069637883008355No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC80.22284122562674097No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC80.22284122562674097No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.22284122562674097No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC70.19498607242339833No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC70.19498607242339833No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC70.19498607242339833No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC70.19498607242339833No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC60.1671309192200557No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC60.1671309192200557No Hit
AGCACTTGATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAGTGCGCT60.1671309192200557No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC60.1671309192200557No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC60.1671309192200557No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC50.1392757660167131No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC50.1392757660167131No Hit
AGCACTTGAATCAACGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC50.1392757660167131No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC50.1392757660167131No Hit
AGCACTTGAATCAACAATATCAGCAACCGAAAGACCAAGTCTCTGCTACC50.1392757660167131No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC40.11142061281337048No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCACTGCTAC40.11142061281337048No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC40.11142061281337048No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC40.11142061281337048No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC40.11142061281337048No Hit
AGCACTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC40.11142061281337048No Hit
AGCACTTAAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC40.11142061281337048No Hit
AGCACTTGAATCAACAATGATCAGAACCGAAAGACCAAGTCTCTGCTACC40.11142061281337048No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC40.11142061281337048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)