FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755
Total Bases829.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC403470.09556907037359No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC4117.141615986099044No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC2694.674196350999131No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC851.4769765421372718No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC671.1642050390964378No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.48653344917463076No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.46915725456125107No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.4170286707211121No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.3996524761077324No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.33014769765421376No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.31277150304083406No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC160.27801911381407474No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC160.27801911381407474No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC150.26064291920069504No Hit
AGCACTTGATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAAGTCATC140.24326672458731538No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC140.24326672458731538No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.24326672458731538No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC130.2258905299739357No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC120.20851433536055605No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC120.20851433536055605No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.1911381407471764No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC110.1911381407471764No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC110.1911381407471764No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC110.1911381407471764No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC100.1737619461337967No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.1737619461337967No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.1737619461337967No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC90.15638575152041703No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC90.15638575152041703No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.15638575152041703No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC90.15638575152041703No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC90.15638575152041703No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC80.13900955690703737No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC80.13900955690703737No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC80.13900955690703737No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC80.13900955690703737No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC70.12163336229365769No Hit
AGCACTTGAATCAACGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC70.12163336229365769No Hit
AGCACTTAAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC70.12163336229365769No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC70.12163336229365769No Hit
AGACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC70.12163336229365769No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC70.12163336229365769No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC60.10425716768027803No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC60.10425716768027803No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATATTCAAACTCACAC60.10425716768027803No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC60.10425716768027803No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC60.10425716768027803No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCTATTTTCAAACTCACAC60.10425716768027803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)