FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755
Total Bases834.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC374965.14335360556038No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA3726.463944396177237No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA2414.1876629018245No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA611.0599478714161599No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.5734144222415292No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.3996524761077324No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.3996524761077324No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT220.3822762814943528No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3475238922675934No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3475238922675934No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC190.33014769765421376No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC190.33014769765421376No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.2953953084274544No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC160.27801911381407474No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.27801911381407474No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.26064291920069504No Hit
TGCTGCTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATGTCCTC150.26064291920069504No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.24326672458731538No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC140.24326672458731538No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC130.2258905299739357No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.2258905299739357No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC130.2258905299739357No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC130.2258905299739357No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.2258905299739357No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.2258905299739357No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC120.20851433536055605No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC110.1911381407471764No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC100.1737619461337967No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC100.1737619461337967No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC90.15638575152041703No Hit
TGCTGATCATTGTTGCTTCGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.15638575152041703No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC90.15638575152041703No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.13900955690703737No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.13900955690703737No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.13900955690703737No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC70.12163336229365769No Hit
TGCTGATCATTGTTGATTCAAGTCTGTGTGATGTAGAACCATGTCGTCAG70.12163336229365769No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCAC70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTAAGTGCTGTGTGATGTAGAACCATGTCGTCA70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC70.12163336229365769No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTACCTC60.10425716768027803No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAATTCCTC60.10425716768027803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)