FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7024
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC348949.67255125284738No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCATACTCACAC164223.376993166287015No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC3434.883257403189066No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC3044.328018223234624No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC711.0108200455580867No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC560.7972665148063782No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCATACTCACAC340.48405466970387245No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.3843963553530752No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC250.3559225512528474No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.3416856492027335No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAT220.31321184510250566No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.31321184510250566No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.2847380410022779No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.22779043280182232No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC150.21355353075170844No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCATACTCACACC150.21355353075170844No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCATACTCACACC140.19931662870159456No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC140.19931662870159456No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC140.19931662870159456No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCTATTTTCAAACTCACAC140.19931662870159456No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.18507972665148065No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC130.18507972665148065No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.18507972665148065No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC130.18507972665148065No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC110.15660592255125283No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC110.15660592255125283No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.14236902050113895No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC100.14236902050113895No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCATACTCACACC100.14236902050113895No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC90.12813211845102507No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCATACTCACACC80.11389521640091116No Hit
AGCACTTGATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAAGAGTCG80.11389521640091116No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC80.11389521640091116No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCATACTCACACC80.11389521640091116No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC80.11389521640091116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)