FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447
Total Bases785.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC348764.01689003120984No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC4698.610244171103359No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC4207.710666421883605No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC971.7807967688635948No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC911.6706443914081146No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC250.45896823939783365No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC250.45896823939783365No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.4406095098219203No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.4406095098219203No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.4038920506700936No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC210.3855333210941803No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.3488158619423536No Hit
AGCACTTGAATCAACAACCGAAAGACCAAGTCTCTGCTACCGTATCGCGT190.3488158619423536No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.3120984027905269No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC160.29373967321461353No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC160.29373967321461353No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC140.25702221406278686No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.23866348448687352No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC130.23866348448687352No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC120.22030475491096016No Hit
AGCACTTGAAGCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC110.2019460253350468No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.18358729575913346No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC100.18358729575913346No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC100.18358729575913346No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC100.18358729575913346No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC90.1652285661832201No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC90.1652285661832201No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.1652285661832201No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC80.14686983660730676No Hit
AGCACTTGAATCAATAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC80.14686983660730676No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC80.14686983660730676No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC80.14686983660730676No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.14686983660730676No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC70.12851110703139343No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC70.12851110703139343No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC70.12851110703139343No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC70.12851110703139343No Hit
AGCACTTGAATCACCAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC70.12851110703139343No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCACTGCTAC60.11015237745548008No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC60.11015237745548008No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC60.11015237745548008No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC60.11015237745548008No Hit
AGACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC60.11015237745548008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)