FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC616274.17840375586854No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC3303.9725532683279163No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC2122.5520645238955098No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC1241.4927169856747322No Hit
GCATTGCCTGGGGATTTGACAGCTGAGGAGGGTCTAGATCCTCTTGACAG841.0111953773925604No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC520.6259780907668232No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA500.6019020103527146No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC420.5055976886962802No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC420.5055976886962802No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC400.4815216082821716No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.4213314072469002No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.34910316600457447No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC260.3129890453834116No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC250.3009510051763573No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC250.3009510051763573No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.27687492476224873No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC230.27687492476224873No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC230.27687492476224873No Hit
AGCACTTGAATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAACATAG220.26483688455519444No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC210.2527988443481401No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC210.2527988443481401No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC210.2527988443481401No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC200.2407608041410858No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.22872276393403151No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC190.22872276393403151No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.22872276393403151No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC180.21668472372697722No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC180.21668472372697722No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.20464668351992293No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC170.20464668351992293No Hit
AGCACTTGATCAACAACCGAAAGACCAAGTCTCTGCTACCGTAACATAGT170.20464668351992293No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC160.19260864331286867No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC160.19260864331286867No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC150.18057060310581438No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC150.18057060310581438No Hit
AGCACTTGAATCAACGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.1564945226917058No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.1444564824846515No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC110.13241844227759722No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.1203804020705429No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC100.1203804020705429No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC90.10834236186348861No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.10834236186348861No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATATTCAAACTCACAC90.10834236186348861No Hit
AGCACTTGAATCAACAGACTTCAGAGACTGAGCCATTTTCAAACTCACAC90.10834236186348861No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)