FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8250
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC564268.38787878787879No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA3534.278787878787878No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA2593.139393939393939No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT640.7757575757575758No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGTCTGGAGTATTTCCTCT440.5333333333333333No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT440.5333333333333333No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA410.49696969696969695No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT370.4484848484848485No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT280.3393939393939394No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC280.3393939393939394No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC240.2909090909090909No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.2909090909090909No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.2909090909090909No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.2787878787878788No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.2787878787878788No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC230.2787878787878788No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC220.26666666666666666No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC210.2545454545454546No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC190.2303030303030303No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.2303030303030303No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC180.2181818181818182No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.20606060606060606No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAAGAAAAGTCTGGAGTATTTCCTC160.19393939393939394No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC160.19393939393939394No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT150.18181818181818182No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC150.18181818181818182No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.18181818181818182No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC150.18181818181818182No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC140.1696969696969697No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT140.1696969696969697No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.1696969696969697No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.1575757575757576No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC130.1575757575757576No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC120.14545454545454545No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTTGAGTATTTCCTC120.14545454545454545No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.14545454545454545No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.14545454545454545No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.13333333333333333No Hit
TGCTGATCATTGTTGCTTTGGGGATGAGGAAAAGTCTGGAGTATTTCCTC110.13333333333333333No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC110.13333333333333333No Hit
TGCTGATCCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.13333333333333333No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.13333333333333333No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC100.12121212121212122No Hit
TGCTGATCATTGTTGCTTTGGGAGTGAGGAAAAGTCTGGAGTATTTCCTC100.12121212121212122No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.1090909090909091No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.1090909090909091No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC90.1090909090909091No Hit
TGCTGATCATTGTTGCTTCGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.1090909090909091No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC90.1090909090909091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)