FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8698
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC607569.84364221660152No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA3834.40331110600138No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA2172.494826396872844No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT520.5978385835824327No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT490.5633478960680617No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT440.5058634168774432No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA430.4943665210393194No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.4023913543343297No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.34490687514371116No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.3334099793055875No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT260.29891929179121635No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.24143481260059785No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.2299379167624741No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.21844102092435044No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC180.20694412508622673No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.20694412508622673No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC170.19544722924810298No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC170.19544722924810298No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC160.18395033340997932No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC160.18395033340997932No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC160.18395033340997932No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC150.17245343757185558No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC140.1609565417337319No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC140.1609565417337319No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.1609565417337319No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC140.1609565417337319No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.1609565417337319No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT130.14945964589560817No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC130.14945964589560817No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.14945964589560817No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC130.14945964589560817No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCATC120.13796275005748448No Hit
TGCTGCTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATGTCCTC120.13796275005748448No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC110.1264658542193608No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1264658542193608No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1264658542193608No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC110.1264658542193608No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.1264658542193608No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC110.1264658542193608No Hit
TGCTGATCATTGTTGCTTTGGGGGAGAGGAAAAGTCTGGAGTATTTCCTC100.11496895838123705No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.11496895838123705No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC100.11496895838123705No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC100.11496895838123705No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC100.11496895838123705No Hit
TGCTGATCATTGTTGTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10347206254311336No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTA90.10347206254311336No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAATTCCTC90.10347206254311336No Hit
TGCTGATCATTGTTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10347206254311336No Hit
AGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10347206254311336No Hit
TGCCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10347206254311336No Hit
TGCTGATGATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10347206254311336No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC90.10347206254311336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)