FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999
Total Bases289.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC95347.67383691845923No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA34317.158579289644823No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20310.155077538769385No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA472.351175587793897No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT100.5002501250625313No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.5002501250625313No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.4502251125562781No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.400200100050025No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.3501750875437719No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.3001500750375188No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.3001500750375188No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC50.25012506253126565No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC50.25012506253126565No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT50.25012506253126565No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT50.25012506253126565No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC50.25012506253126565No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC40.2001000500250125No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTA40.2001000500250125No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT40.2001000500250125No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAAGAAAAGTCTGGAGTATTTCCTC40.2001000500250125No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC30.1500750375187594No Hit
TGCTGATGATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.1500750375187594No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.1500750375187594No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC30.1500750375187594No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.1500750375187594No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC30.1500750375187594No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.1500750375187594No Hit
TGCTGATCATTGTTGATTCAAGTGCGTGTGATGTAGAACCATGTCGTCAG30.1500750375187594No Hit
TGCTGATCATTGTTGATTCAAGTCTGTGTGATGTAGAACCATGTCGTCAG30.1500750375187594No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC30.1500750375187594No Hit
TGCTGATCATTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTT30.1500750375187594No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.1500750375187594No Hit
TGCTGCTCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGGCGTCA20.10005002501250625No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC20.10005002501250625No Hit
TGCTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT20.10005002501250625No Hit
TGCTGATCATTTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC20.10005002501250625No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTAA20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC20.10005002501250625No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC20.10005002501250625No Hit
TGCTGAATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCTT20.10005002501250625No Hit
TGCTGATATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
AGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGTGATGTAGAACCATGTCGTCAGTGTGTTGA20.10005002501250625No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC20.10005002501250625No Hit
TGCTGATGATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.10005002501250625No Hit
TGCTGATCATTGATGCTATGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC20.10005002501250625No Hit
TGCTGATCATTGTTGATTCAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTGATCATTGTTGTTTCAAGTGCTGTGTGATGTAGAAGCATGTCGTCA20.10005002501250625No Hit
TGCTGATCATTGTTGCTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC20.10005002501250625No Hit
TGCCGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGACTGGAGTATTTCCTC20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGAAGTATTTCCTC20.10005002501250625No Hit
TGCTGATCATTGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC20.10005002501250625No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGAAGAACCATGTCGTCA20.10005002501250625No Hit
GCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTGTCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.10005002501250625No Hit
TGCTGATCCTTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCC20.10005002501250625No Hit
TGCTGATCAATGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.10005002501250625No Hit
TGCAGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC20.10005002501250625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)