FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4837
Total Bases697.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC318565.8465991316932No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC63413.10729791192888No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC2595.354558610709118No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC631.3024602026049203No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC350.723589001447178No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC200.41347942939838744No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.39280545792846805No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.3721314864585487No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.3514575149886293No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.26876162910895185No Hit
AGCACTTGAATCAACAACCGAAAGACCAAGTCTCTGCTACCGTACTCTTA130.26876162910895185No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.26876162910895185No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.24808765763903248No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.24808765763903248No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.20673971469919372No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC90.18606574322927436No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC90.18606574322927436No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC80.16539177175935496No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC70.1447178002894356No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC70.1447178002894356No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC70.1447178002894356No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC60.12404382881951624No Hit
AGCACTTGAAGCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC60.12404382881951624No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC60.12404382881951624No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC60.12404382881951624No Hit
AGCACTTGATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC60.12404382881951624No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATATTCAAACTCACAC60.12404382881951624No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC60.12404382881951624No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC60.12404382881951624No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCACTGCTAC50.10336985734959686No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC50.10336985734959686No Hit
AGCATTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC50.10336985734959686No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGTGCCATTTTCAAACTCACAC50.10336985734959686No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC50.10336985734959686No Hit
AGCACTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTACC50.10336985734959686No Hit
AGCACTTGAATCAACAGGCAGCACAAACACAACCATCCCAGAGTTCACAT50.10336985734959686No Hit
AGCACTTGATCAGCAACCGAAAGACCAAGTCTCTGCTACCGTACTCTTAG50.10336985734959686No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC50.10336985734959686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)