FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT338048.74531295067782No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130918.877992500721085No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA3334.802422843957312No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA2643.807326218632824No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA500.7210845111047015No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.3893856359965388No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC240.3461205653302567No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT230.3316988751081627No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC180.25959042399769255No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.24516873377559853No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.24516873377559853No Hit
TGCTGATCATTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC160.23074704355350445No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT160.23074704355350445No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.2019036631093164No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.2019036631093164No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT140.2019036631093164No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT130.18748197288722238No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT130.18748197288722238No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.18748197288722238No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.18748197288722238No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTCCCT120.17306028266512835No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT120.17306028266512835No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT120.17306028266512835No Hit
TGCTGATCATAGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT120.17306028266512835No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.15863859244303433No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT110.15863859244303433No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAGAAGTCTGGAGTATTTCCT110.15863859244303433No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC100.1442169022209403No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC100.1442169022209403No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1442169022209403No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1442169022209403No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.1442169022209403No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGGGTATTTCCT90.12979521199884628No Hit
TGCTGATCATTGTTGTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.12979521199884628No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.12979521199884628No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.12979521199884628No Hit
TGCTGATCAATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT80.11537352177675222No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTACT80.11537352177675222No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT80.11537352177675222No Hit
TGCTGATCATTGTTGCTTTGGGGGGAGAGGAAAAGTCTGGAGTATTTCCT80.11537352177675222No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT70.1009518315546582No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT70.1009518315546582No Hit
TGCTGATCATTGTTGCTTTGGTGGGTGAGGAAAAGTCTGGAGTATTTCCT70.1009518315546582No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCG70.1009518315546582No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCC70.1009518315546582No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTTTTTCCT70.1009518315546582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)