FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7750
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT375448.438709677419354No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC141518.258064516129032No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA5236.748387096774193No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA1942.503225806451613No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAATATTTCCT1021.3161290322580645No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA570.7354838709677419No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC310.4No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT310.4No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC280.36129032258064514No Hit
TGCTGATCATTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC280.36129032258064514No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC200.25806451612903225No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC190.24516129032258063No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC180.23225806451612901No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.21935483870967742No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.21935483870967742No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.2064516129032258No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.18064516129032257No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT140.18064516129032257No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.16774193548387098No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT120.15483870967741936No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.15483870967741936No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.14193548387096774No Hit
TGCGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.14193548387096774No Hit
TGCTGATCATTTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.12903225806451613No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT100.12903225806451613No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT100.12903225806451613No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGGGTATTTCCT90.11612903225806451No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGTAGTATTTCCT90.11612903225806451No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC90.11612903225806451No Hit
TGCTGATCATTGTTGATTCAAGTCTGTGTGATGTAGAACCATGTCGTCAG90.11612903225806451No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT90.11612903225806451No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT90.11612903225806451No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.11612903225806451No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTACCT80.1032258064516129No Hit
TGCTGCTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTAGTTCCT80.1032258064516129No Hit
TGCTGATCATTGTTGCTTTGGGGAGTGAGGAAAAGTCTGGAGTATTTCCT80.1032258064516129No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTGCT80.1032258064516129No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT80.1032258064516129No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT80.1032258064516129No Hit
TGCTGATCATAGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT80.1032258064516129No Hit

[FAIL]Adapter Content

Adapter graph