FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC534573.65302466583988No Hit
AGCACTTGAATCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC4345.980432685682789No Hit
AGCACTTGAAGCAACAATGATCAGCAACCGAAAGACCAAGTCTCTGCTAC2733.761885076477883No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC971.3366404850489182No Hit
AGCACTTGAATCAACAGTGATCAGCAACCGAAAGACCAAGTCTCTGCTAC470.6476505443020533No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC320.4409535620779937No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.4409535620779937No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.3996141656331818No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC280.38583436681824446No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.37205456800330716No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.37205456800330716No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.31693537274355793No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC200.275595976298746No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC180.24803637866887143No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC180.24803637866887143No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.24803637866887143No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC170.23425657985393414No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC160.22047678103899684No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC160.22047678103899684No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC160.22047678103899684No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC150.20669698222405952No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC150.20669698222405952No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.19291718340912223No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC140.19291718340912223No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.19291718340912223No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC130.17913738459418493No Hit
AGCACTTGAATCAACAACCGAAAGACCAAGTCTCTGCTACCGTACCATAT130.17913738459418493No Hit
AGCACTTGAAGCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC120.1653575857792476No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.15157778696431032No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC110.15157778696431032No Hit
AGCACTTGAATCACCAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC110.15157778696431032No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC110.15157778696431032No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC100.137797988149373No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC100.137797988149373No Hit
AGCGCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.137797988149373No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC100.137797988149373No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC80.11023839051949842No Hit
AGCACTTGATCAACAACCGAAAGACCAAGTCTCTGCTACCGTACCATATG80.11023839051949842No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC80.11023839051949842No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATATTCAAACTCACAC80.11023839051949842No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC80.11023839051949842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)