FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630
Total Bases236.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC71743.987730061349694No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA25115.39877300613497No Hit
TGCTGATCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA17310.613496932515337No Hit
TGCTGATCACTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA452.7607361963190185No Hit
TGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCT110.6748466257668712No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.49079754601227No Hit
TGTTGATCAAGTGCTGTGTGATGTAGAACCATGTCGTCAGTGTAGATCTC70.4294478527607362No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.36809815950920244No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT60.36809815950920244No Hit
TGCTGATCACTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA50.3067484662576687No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC50.3067484662576687No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC50.3067484662576687No Hit
TGCTGATCATTGTTGCTTTAAGTGCTGTGTGATGTAGAACCATGTCGTCA50.3067484662576687No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC40.245398773006135No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT40.245398773006135No Hit
TGCTGATCATTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTT40.245398773006135No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC40.245398773006135No Hit
TGCTGATCATTGTTGCTTCGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.18404907975460122No Hit
TGCTGTTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.18404907975460122No Hit
TGCTGATCATTGTTGCTTTGGGGGAGAGGAAAAGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG30.18404907975460122No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.18404907975460122No Hit
TGCTGATCATTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT30.18404907975460122No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC30.18404907975460122No Hit
TGCTGATCATTGTTGCTATGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT30.18404907975460122No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC30.18404907975460122No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.1226993865030675No Hit
TGCTGATCATTTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTC20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC20.1226993865030675No Hit
TGCTGATCATTGTTGTTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGTCATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGCTGATATTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGCTGATCTTGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGCTGATCATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTTA20.1226993865030675No Hit
TGCTGATCATAGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGTGGAAAAGTCTGGAGTATTTCCTC20.1226993865030675No Hit
TGCTGATCAATGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC20.1226993865030675No Hit
TGCTGATCATAGTTGCTTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC20.1226993865030675No Hit
TGCTGACATTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC20.1226993865030675No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.1226993865030675No Hit
TGCTGATCGTTGTTGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGATCATTGATGATTCAAGTGCTGTGTGATGTAGAACCATGTCGTCA20.1226993865030675No Hit
TGCTGATCATTGTTGATTCAAGTCTGTGTGATGTAGAACCATGTCGTCAG20.1226993865030675No Hit
TGCTGATCCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC20.1226993865030675No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT20.1226993865030675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)