FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8517
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC573167.2889515087472No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA2923.4284372431607375No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA2783.2640601150639896No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT891.044968885757896No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT490.5753199483386169No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.49313138429024306No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT410.48139016085476105No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT390.4579077139837971No Hit
TGCTGATCACTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA370.43442526711283314No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC360.42268404367735113No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC320.37571914993542327No Hit
TGCTGATCATTGTTGCTTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTC320.37571914993542327No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT310.3639779264999413No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.3522367030644593No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.3170130327580134No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC250.29353058588704944No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC240.28178936245156744No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC220.2583069155806035No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC210.24656569214512153No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.23482446870963955No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC190.2230832452741576No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT190.2230832452741576No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC190.2230832452741576No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC190.2230832452741576No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.2230832452741576No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC180.21134202183867556No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.21134202183867556No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC170.19960079840319359No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.18785957496771163No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC150.17611835153222966No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.17611835153222966No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC140.16437712809674768No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.16437712809674768No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.16437712809674768No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.1526359046612657No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12915345779030174No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.12915345779030174No Hit
TTGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.12915345779030174No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC110.12915345779030174No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGACTGGAGTATTTCCTC110.12915345779030174No Hit
TGCTGATCATTGTTGCTATGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12915345779030174No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC110.12915345779030174No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC100.11741223435481977No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC100.11741223435481977No Hit
TGCTGATCATTGTTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATC100.11741223435481977No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10567101091933778No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.10567101091933778No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTACCTC90.10567101091933778No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC90.10567101091933778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)