FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7232
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC486467.2566371681416No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA1902.627212389380531No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA1702.350663716814159No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1051.4518805309734513No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT470.6498893805309734No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT440.6084070796460177No Hit
CTCATCCTTGTCCACCGAAGATCCCTTTGTCACTGCAGCTTCTCTAGTGT420.5807522123893806No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.4701327433628319No Hit
TGCTGATCACTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA320.4424778761061947No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT270.3733407079646018No Hit
TGCTGATCATTGTTGCTTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.3733407079646018No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC250.3456858407079646No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC240.33185840707964603No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.33185840707964603No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.33185840707964603No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.31803097345132747No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT230.31803097345132747No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC210.2903761061946903No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC210.2903761061946903No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC210.2903761061946903No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.27654867256637167No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.27654867256637167No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC190.2627212389380531No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC190.2627212389380531No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC180.2488938053097345No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC170.23506637168141595No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.22123893805309736No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC160.22123893805309736No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.22123893805309736No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.2074115044247788No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC140.19358407079646017No Hit
TGCTGATCATTGTTGCTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT140.19358407079646017No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.19358407079646017No Hit
TTGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT140.19358407079646017No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC140.19358407079646017No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC130.17975663716814158No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.17975663716814158No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.17975663716814158No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTTGAGTATTTCCTC110.15210176991150443No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC110.15210176991150443No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT100.13827433628318583No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC100.13827433628318583No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCAC100.13827433628318583No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC100.13827433628318583No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC100.13827433628318583No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.13827433628318583No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGAAAAAGTCTGGAGTATTTCCTC90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC90.12444690265486726No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGAGTGAGGAAAAGTCTGGAGTATTTCCTC90.12444690265486726No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTACCTC80.11061946902654868No Hit
TGCTGATCATTGTTGCTATGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.11061946902654868No Hit
TGCTGATCATTGTTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATC80.11061946902654868No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)