FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8795
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC673376.55486071631609No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC1391.580443433769187No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC1171.3303013075611143No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1161.3189312109152929No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC1031.1711199545196136No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC750.8527572484366117No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC610.6935758953951109No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC500.5685048322910745No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTGACG450.511654349061967No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC430.4889141557703241No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.44343376918703814No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC370.42069357589539513No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC360.4093234792495736No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC290.3297328027288232No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.3069926094371802No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC260.2956225127913587No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC260.2956225127913587No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC260.2956225127913587No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC250.28425241614553726No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC250.28425241614553726No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC220.25014212620807275No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC210.2387720295622513No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC200.22740193291642977No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC200.22740193291642977No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC190.21603183627060832No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC190.21603183627060832No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC190.21603183627060832No Hit
AGCACTTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA180.2046617396247868No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC180.2046617396247868No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC170.19329164297896534No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC170.19329164297896534No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC170.19329164297896534No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC150.17055144968732233No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.15918135304150086No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC140.15918135304150086No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTGACGT140.15918135304150086No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC130.14781125639567935No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC120.13644115974985788No Hit
AGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTGAC120.13644115974985788No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC120.13644115974985788No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC120.13644115974985788No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.12507106310403637No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC110.12507106310403637No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC100.11370096645821488No Hit
AGCACTTGAATTAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.11370096645821488No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTTACAC100.11370096645821488No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.11370096645821488No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC90.1023308698123934No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC90.1023308698123934No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCATAC90.1023308698123934No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCTCAC90.1023308698123934No Hit
AACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.1023308698123934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)