FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008125993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008125993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC658970.8266150704074No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1581.6983768676770934No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA1261.3544018058690745No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA860.9244329786090508No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT520.5589594754380307No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT520.5589594754380307No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC430.4622164893045254No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC370.3977211652155219No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC360.3869719445340213No Hit
TGCTGATCATTGTTGCTTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTC360.3869719445340213No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.3762227238525207No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.3654735031710201No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.3547242824895195No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.3547242824895195No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC300.32247662044501774No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT290.31172739976351715No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC290.31172739976351715No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC260.27947973771901535No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC250.26873051703751477No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT250.26873051703751477No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT250.26873051703751477No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC240.2579812963560142No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC240.2579812963560142No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC220.236482854993013No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.2257336343115124No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC210.2257336343115124No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC200.2149844136300118No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC200.2149844136300118No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC190.20423519294851122No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.20423519294851122No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC190.20423519294851122No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC180.19348597226701064No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC180.19348597226701064No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.19348597226701064No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.18273675158551006No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC170.18273675158551006No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.17198753090400945No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.16123831022250887No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC150.16123831022250887No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.16123831022250887No Hit
TGCTGATCACTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA150.16123831022250887No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.16123831022250887No Hit
TTGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT140.15048908954100826No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.1289906481780071No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC120.1289906481780071No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1182414274965065No Hit
TGCTGATCATTGTTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.1182414274965065No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC110.1182414274965065No Hit
TGCTGATCATTGTTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATC110.1182414274965065No Hit
TGCTGATCATTGTTGTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1074922068150059No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTAGAGTATTTCCTC100.1074922068150059No Hit
TGCAGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1074922068150059No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1074922068150059No Hit
TGCTGATCATTGTTGCTTTGGGTGTGAGGAAAAGTCTGGAGTATTTCCTC100.1074922068150059No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC100.1074922068150059No Hit
TGCTGACCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.1074922068150059No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)