FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9951
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC715871.93246909858306No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1901.9093558436338056No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA930.9345794392523363No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA510.5125113053964425No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT510.5125113053964425No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT500.5024620641141594No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT450.4522158577027435No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC420.4220681338558939No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT420.4220681338558939No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC400.4019696512913275No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT390.39192041000904426No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.3416742035976284No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC330.33162496231534516No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT320.32157572103306203No Hit
TGCTGATCATTGTTGCTTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTC290.29142799718621243No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC280.28137875590392925No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC270.27132951462164606No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.2612802733393629No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC260.2612802733393629No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC260.2612802733393629No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC250.2512310320570797No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC250.2512310320570797No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT250.2512310320570797No Hit
TGCTGATCATTGTTGCTTTGGTGGTGAGGAAAAGTCTGGAGTATTTCCTC240.2411817907747965No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.22108330821023012No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.21103406692794696No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.21103406692794696No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT210.21103406692794696No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC200.20098482564566375No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC190.19093558436338057No Hit
TTGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT180.18088634308109736No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC170.1708371017988142No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC170.1708371017988142No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC160.16078786051653102No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC160.16078786051653102No Hit
TGCTGATCATTGTTGCTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT150.1507386192342478No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCGTC150.1507386192342478No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.1507386192342478No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATATCCTC140.14068937795196462No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.14068937795196462No Hit
TGCTGATCACTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA140.14068937795196462No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.14068937795196462No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.13064013666968144No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.13064013666968144No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC120.12059089538739826No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAATTCCTC120.12059089538739826No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.12059089538739826No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC120.12059089538739826No Hit
TGCCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.12059089538739826No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC120.12059089538739826No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTACTC110.11054165410511506No Hit
TGCAGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.11054165410511506No Hit
TGCTGATCATTGTTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.11054165410511506No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC110.11054165410511506No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGCGTATTTCCTC110.11054165410511506No Hit
TGCTGATCATTGTTACTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.10049241282283188No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.10049241282283188No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTACCTC100.10049241282283188No Hit
TGCTGACCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.10049241282283188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)