FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7911
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC606376.64012135001896No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC1892.3890784982935154No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC1451.832890911389205No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC981.2387814435596005No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC971.2261408165845027No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAATCGCT580.733156364555682No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC540.6825938566552902No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC460.5814688408545065No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.37921880925293894No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.37921880925293894No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.366578182277841No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.3412969283276451No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC250.3160156743774491No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC240.30337504740235116No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC230.2907344204272532No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC220.27809379345215524No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC210.26545316647705725No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC210.26545316647705725No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC210.26545316647705725No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC200.2528125395019593No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC200.2528125395019593No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC190.24017191252686132No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC190.24017191252686132No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC180.22753128555176336No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.22753128555176336No Hit
AGCACTTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA160.20225003160156746No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC160.20225003160156746No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC150.18960940462646947No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC150.18960940462646947No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC140.1769687776513715No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC130.16432815067627354No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC130.16432815067627354No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC130.16432815067627354No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC120.15168752370117558No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC120.15168752370117558No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC120.15168752370117558No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC110.13904689672607762No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC110.13904689672607762No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC110.13904689672607762No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC100.12640626975097966No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC100.12640626975097966No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAATCGCTA100.12640626975097966No Hit
AGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAATCGC100.12640626975097966No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC100.12640626975097966No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCTATTTTCAAACTCACAC100.12640626975097966No Hit
AGCACTTGAATCAACGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC90.11376564277588168No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTTACAC90.11376564277588168No Hit
AGCACTTGAATCAACAGGCTTTAGAGACTGAGCCATTTTCAAACTCACAC90.11376564277588168No Hit
GGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC80.10112501580078373No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC80.10112501580078373No Hit
AGCACTTGAATCAAAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC80.10112501580078373No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)