FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616
Total Bases89.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC19631.818181818181817No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC14824.025974025974026No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC12219.805194805194805No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC233.7337662337662336No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTA111.7857142857142856No Hit
AGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGT91.461038961038961No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTAA81.2987012987012987No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC60.974025974025974No Hit
AGACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC30.487012987012987No Hit
AGCACTTGAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC30.487012987012987No Hit
AGCACTTGAATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTA30.487012987012987No Hit
AGCACTTGAATCGACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC20.3246753246753247No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.3246753246753247No Hit
AGCACTTGAAGCAACAATGACAGCAAAGACGAGACCAAGTCTCTGCTACC20.3246753246753247No Hit
AGCATTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC20.3246753246753247No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGACTCTGCTAC20.3246753246753247No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC20.3246753246753247No Hit
AGCACTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC20.3246753246753247No Hit
AGCACTTGAAGCAACAATGATCACAAAGACGAGACCAAGTCTCTGCTACC20.3246753246753247No Hit
AGCACTTGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTAA20.3246753246753247No Hit
GCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC20.3246753246753247No Hit
AGCACTTGAATCAACAATGATCAGCAAGACGAGACCAAGTCTCTGCTACC20.3246753246753247No Hit
AGCACTTGAAGCAACAATGATCAACAAAGACGAGACCAAGTCTCTGCTAC20.3246753246753247No Hit
AGCACTTGAATCAACAAAGATCAGCAAAGACGAGACCAAGACACTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAAAAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGCCCAAGTCACTGCTAC10.16233766233766234No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTATGCTAAC10.16233766233766234No Hit
AGCACTTGAAGCAACATTGATCAGCTAAGACGAGACGATGTCTCTGCAAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCAACAATGATAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTA10.16233766233766234No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGCACTTAAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACGAACTCACAC10.16233766233766234No Hit
AGCACTTGAACAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
ACACTTGAATCAACAATGATCATCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
AGCACTTGAATCAACAATGATAAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAGTGATCAACAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
GCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTAA10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAGCAACAATGATCAGCAAAGACGAGACCAAGTCTC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTCGACATGGTTCTACACACCAAAGCAACAAT10.16233766233766234No Hit
AGCACTGAATCAACAGGCTTCAGAGACTAAGCCATTTTCAAACTCGCACC10.16233766233766234No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCTAGTCTCTGCTAC10.16233766233766234No Hit
AGCATTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
AGCACTTGTAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAAGCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTA10.16233766233766234No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
GACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCG10.16233766233766234No Hit
AGCACTTATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCG10.16233766233766234No Hit
AGCACTAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCG10.16233766233766234No Hit
AGCACTTGAATCAACAAAGACGAGAAAAGCAACAATGATCAGCAAAGACG10.16233766233766234No Hit
GCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
AGCACTTGAATCAACAGACTTCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGTACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACACC10.16233766233766234No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGCACTAGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
GGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCAGCACAGACTGAGCCATAAACAAACACACAC10.16233766233766234No Hit
AGCACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTAGTCGTA10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC10.16233766233766234No Hit
ACACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTCTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGAATCAACAATGTTCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAAGCAACAATGATAGCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
AGCCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGTACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC10.16233766233766234No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.16233766233766234No Hit
AGCACTTGAATCACACTGACGACATGGTTCTACACACGAAAGCACTTGAA10.16233766233766234No Hit
AGCACTTGAATCAACAATATCAGCAAAGACGAGACCAAGTCTCTGCTACC10.16233766233766234No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCAGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACATTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACAAAGTCTCTGCTAC10.16233766233766234No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCACTGCTAC10.16233766233766234No Hit
AGCACTTGAAACAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC10.16233766233766234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)